AeroPod, PowerPod and Newton

Thank you, So when I have paired the sensors for bike 1 (or 2) can I go immediately on the O/B calibration ride?
I don't need to connect to Isaac after the paring before the ride?

Would you please advise on how to set up two bikes with the BEST accuracy settings.
I have just purchased a new powerpod so will be setting this up from scratch, ie nothing is yet paired to the pod